Determination of protein degradability of alfalfa hay via buffer or protease

In vitro determination of ruminal protein degradability of alfalfa and prairie hay via a commercial protease in the presence or absence of cellulase or driselase.

Ruminal protein degradation of alfalfa (2.62% N, 49.6% NDF, and in vivo undegradable intake protein [UIP] = 16.4% of CP) and prairie hay (.88% N, 69.4% NDF, and in vivo UIP = 44.5% of CP) was estimated using the Streptomyces griseus protease (SGP) in vitro method with or without pretreatment with two carbohydrases: cellulase from Penicillium funiculosum or driselase from Basidiomycetes. Drisela...

Ruminal in vitro degradability of protein in alfalfa harvested as standing forage or baled hay.

Eighty-nine samples, 45 of standing forage and 44 of baled hay, were collected from alfalfa harvested at various maturities over three cuttings each during 2 yr. Alfalfa was cut and conditioned mechanically; samples of standing forage were collected by removing bunches of forage from windrows and freeze-drying them. Forage was allowed to field cure and was harvested at an average 80% DM as smal...

Effect of yeast culture supplementation of alfalfa silage or alfalfa hay fed to lactating dairy cows

Synthesis of microbial protein in ruminally cannulated cows fed alfalfa silage, alfalfa hay, or corn silage.

Six ruminally cannulated cows were used in an experiment with a 3 x 3 Latin square design. Three all forage diets-alfalfa silage, alfalfa hay, or corn silage plus 2.2% urea (DM basis)-were fed for ad libitum intake four times daily. The microbial protein marker 15NH3 and the liquid marker Cr-EDTA were infused continuously into the rumen for 72 and 48 h, respectively; the solid marker, Yb-labele...

Effect of Dry Matter Content and Inoculation on Ruminal Protein Degradability in Alfalfa Silages

The aim of the experiment was to determine the effect of different dry matter content and addition of silage inoculant on the ruminal protein degradability. Alfalfa herbage was collected from two cuts and the fresh matter was sampled two times during each cut (with different dry matter content). During the preparation of experimental micro-silages, the chopped forage was treated with a microbia...